Temperature dependence of mating-pair formation in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Temperature-dependence of the single-cell variability in the kinetics of transcription activation in Escherichia coli.
From in vivo single-cell, single-RNA measurements of the activation times and subsequent steady-state active transcription kinetics of a single-copy Lac-ara-1 promoter in Escherichia coli, we characterize the intake kinetics of the inducer (IPTG) from the media, following temperature shifts. For this, for temperature shifts of various degrees, we obtain the distributions of transcription activa...
متن کاملAccounting for mating pair formation in plasmid population dynamics.
Plasmids are important vehicles for horizontal gene transfer and rapid adaptation in bacteria, including the spread of antibiotic resistance genes. Conjugative transfer of a plasmid from a plasmid-bearing to a plasmid-free bacterial cell requires contact and attachment of the cells followed by plasmid DNA transfer prior to detachment. We introduce a system of differential equations for plasmid ...
متن کاملConjugative junctions in RP4-mediated mating of Escherichia coli.
The physical association of bacteria during conjugation mediated by the IncPalpha plasmid RP4 was investigated. Escherichia coli mating aggregates prepared on semisolid medium were ultrarapidly frozen using copper block freezing, followed by freeze substitution, thin sectioning, and transmission electron microscopy. In matings where the donor bacteria contained conjugative plasmids, distinctive...
متن کاملTemperature dependence of MinD oscillation in Escherichia coli: running hot and fast.
We observed that the oscillation period of MinD within rod-like and filamentous cells of Escherichia coli varied by a factor of 4 in the temperature range from 20 degrees C to 40 degrees C. The detailed dependence was Arrhenius, with a slope similar to the overall temperature-dependent growth curve of E. coli. The detailed pattern of oscillation, including the characteristic wavelength in filam...
متن کاملTemperature dependence of sex-factor maintenance in Escherichia coli K-12.
Wild-type sex factors of Escherichia coli K-12 can be cured from host cells by growth at temperatures above 42 C. This phenomenon is obscured by reinfection of cured cells if the culture is allowed to reach cell densities above 10(5)/ml. The rate of episome loss is dependent on the genetic (chromosomal) background of the host.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1976
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.126.1.222-224.1976